Chapter 81 Centrifugation
"Little Doctor Li?"
The old housekeeper of the Duke of Yan's mansion looked at Li Ang who came to visit suddenly with a look of astonishment.
"How is Duke Yan?"
Li Ang said: "I found a way."
"plz follow me."
The old butler suddenly became serious, and led Li Ang to the lobby.
Along the way, you can still see medicine pots and smell the lingering smell of medicine.
The descendants of the Yan family, including Yan Lin, were standing in the lobby, looking helplessly at Yan Yundang who was sitting with a golden sword on his horse—
His face was still yellow, with his eyes closed, he listened to what the priest-robed man in front of him was saying.
".The general was born in Shuozhou, so naturally the fallen leaves should return to their roots. In the northwest of Shuozhou, there are two mountains, one is Pingding Xingshan and the other is Liaoshan. There is a ditch in the middle called Dadi. The two mountains and one ditch store wind and absorb air. The so-called Qi Cheng The wind will disperse, and the boundary water will stop."
Okay, this is already looking at the feng shui of the cemetery.
Li Ang complained from the bottom of his heart, but it wasn't very strange.
The average life expectancy of Yu people is not high, and the age of seventy years is already very rare. Most elderly people will ask their families to prepare coffins, shrouds and cemeteries early to prevent them from rushing away and being buried in an unseemly way.
The reason why Yan Lin and others were sad was entirely because Yan Yundang, as a master of martial arts, fell ill too suddenly and did not give them any psychological preparation.
The housekeeper stepped forward lightly, and said softly, "Mr. Li, Doctor Li is here."
"Um?"
Yan Yundang opened his eyes, looked back at Li Ang, and said with a smile: "Li Xiaolang, what you said last time is really good. Those pigeons will get sick if they only eat polished rice, but they will recover after eating brown rice.
It seems that I really have a stomach problem, and I can't absorb the strange substance you mentioned. "
"Duke Yan,"
Li Ang cupped his hands and said: "This junior is here for this purpose—I have found a new method, which may relieve the pain."
"What?!"
Before Yan Yundang said anything, Yan Lin took two steps forward with an excited face, "Doctor Li, do you really have a solution?"
Li Ang nodded and said, "It's just a method. The probability of a successful cure is about 70%."
Yan Yundang's jaundice symptoms did not subside, which proved that pernicious anemia was not caused by lack of folic acid. But because of vitamin b12.
"70%"
Yan Lin turned his head and glanced at Yan Yundang. During this time, the Yan Mansion invited many famous doctors and prescribed some medicines including gastrointestinal treatment, but Yan Yundang's pernicious anemia still failed to improve.
"Father, you might as well give it a try."
"Um,"
Yan Yundang took a deep breath. Although he was ready to face death, if he could live one more day and watch his children and grandchildren grow up, he would be able to accept it. prescription"
"Guogong, this medicine does not require a prescription."
Li Ang said: "What I need is fresh pork liver, the more the better;
Cooled and boiled cold water, the more the better;
Several bottles of pure wine produced by Xuegong;
Four ceramic mortars; four thin balsa wood boards cut into circles; sixteen strong strings;
A drilling awl; eight chopsticks;
Four fine cloth bags;
A casserole and a porcelain bowl;
A cart of white clay;
There are also thirty-six glass tubes—basically like this. "
Li Ang asked the housekeeper for a pen and paper, and drew the top view, side view, and front view of the small glass test tube on the paper, and marked the dimensions on the three pictures.
Li Ang asked: "Can this kind of glass pipe be made with Chang'an's craftsman level?"
"able."
Yan Lin glanced at it, then got up and said to Li Ang: "In the best glass workshops and Liuli workshops in Chang'an, there are enshrined masters who can use the power of thoughts to change the shape of hot glass and make it into exquisite utensils."
"That's good."
Li Ang breathed a sigh of relief, "After all these are prepared, we can start making medicines. By the way, General Yan, we still need four strong men and a light-tight room."
"good."
Yanlin immediately asked his subordinates to prepare—although these things are irrelevant, after seeing hundreds of pigeons get sick and recover according to Li Ang's experimental method,
He can only trust Li Ang.
"Little Doctor Li,"
All the family members of the Yan Mansion got busy, and Yan Yundang sat calmly on the seat, and asked a little curiously: "Isn't montmorillonite white clay used for diarrhea? It takes a whole car?"
Li Ang shook his head, "This time it's not antidiarrheal, but filtering."
It was related to Yan Yundang's illness. The entire Yan State government was operating at full capacity, and everything needed for Li Ang was quickly prepared.
"I need to prepare medicine in a light-tight room, please cover the windows with straw mats outside.
If you want to watch from the sidelines, you can enter the house together, but you can only light one oil lamp. "
Li Ang said something casually, and asked the servants of the Yan Mansion to enter the sealed room with all the utensils.
"The dosage of this medicine is quite large. I will only demonstrate it once. Everyone, please keep it in mind. It will be up to you to prepare the medicine for Duke Yan in the future."
Li Ang said to the servants of the Yan Mansion in the room, and with the light of the oil lamp in Yan Lin's hand,
First wash your palms with pure wine, and then wash the fresh pork liver with pure wine to remove the blood stains on it.
Then pick up the scissors, cut the pork liver into pieces, put it into the mortar, grind it quickly and carefully, add water from time to time, and further grind it into crumbs.
The significance of this step is to crush the pork liver rich in vitamin B12, and the next step is to centrifuge.
‘The centrifugation procedure in a normal laboratory is to pour the pig liver fragment solution into a centrifuge tube and put it in a centrifuge. Spin for ten minutes at 3500 rpm.
Separate the solid debris and liquid in the pig liver solution. Obtain a supernatant with a higher vitamin B12 content.
And in the absence of a modern centrifuge, only one can be made.’
Li Ang took the awl, and drilled four diagonally distributed button-shaped holes in the middle of the light circular wooden board, passed two strings through the holes and looped them back, and fixed them on the chopsticks on both sides.
Yanlin asked doubtfully, "This is."
"Children's toys with centrifugal function, rotating flywheel."
Li Ang picked up two chopsticks with a smile, shook the string lightly, pulled and retracted the chopsticks, and the circular wooden board in the middle immediately rotated.
Nice and smooth rotation.
Li Ang put down the flywheel toy with satisfaction, poured the pig liver solution into glass tubes of the same size, plugged them with cork stoppers, and filled eight glass tubes in total.
And each glass tube is firmly tied to both sides of the circular flying disc by means of ropes piercing and fixing holes on the wooden board.
Achieves the same effect as a laboratory centrifuge balance weight.
Next.
Li Ang picked up the flywheel toy, and held the chopsticks to pull and retract, so that the circular wooden disk kept rotating, faster and faster.
This is the most primitive and simple artificial centrifuge in Li Ang's memory in another world, but the effect is not bad.
The centrifuge was invented by Saad Bhamla, a bioengineer at Georgia Tech.
Conventional centrifuges rotate the drum to generate centrifugal force, which separates the fluid into layers in the sample tube by density. All centrifuges require electricity to operate and are bulky and difficult to transport.
This artificial centrifuge, which imitates the principle of children's toys, can reach a maximum speed of thousands or even 10,000 with only ropes and plastics. It is the fastest rotating object driven by manpower. And it doesn't need electricity at all.
The original intention of the invention of the artificial centrifuge is to solve the problem of blood testing in Africa, to separate plasma in the shortest possible time, and to identify whether there is Plasmodium in the blood.
But now, it can also be used to centrifuge vitamin B12 in pork liver solution.
Li Ang pulled the wheel without haste, and asked the other four servants to imitate himself, breaking the pig liver and centrifuging.
Ten minutes later, Li Ang stopped pulling the wheel, and took off the eight glass test tubes on both sides of the wooden disc,
Pour all the supernatant liquid into the casserole, and pour a small amount of white clay into it, stirring constantly.
Clay has a large specific surface area and pore volume, has special adsorption capacity and ion exchange performance, and can adsorb vitamin B12.
Wait until the clay has almost absorbed the solution, Li Ang puts the clay into a clean fine cloth bag, and drains the water.
Then pour the white clay that has absorbed vitamin B12 into a porcelain bowl, add water, and stir vigorously for 20 minutes.
Finally, filter and pour out the stirred solution,
A vitamin B12 solution with a higher content was obtained—because vitamin B12 is easily decomposed when exposed to light, it must be carried out in a dark room.
"finished."
Lian Ang looked at dozens of pieces of pig liver, which was less than a bottle of clear liquid, heaved a sigh of relief, and said to Yan Yundang: "This is the special substance extracted from pig liver.
This substance can relieve anemia and promote development—it can be given to poultry and livestock first, mixed with bran for long-term feeding, and it should be able to promote the rapid growth of livestock to observe the effect. "
"No."
Yan Yundang is also very straightforward. After reading his set of procedures, he can see clearly that Li Ang didn't add anything special to the solution.
Only the pig liver, water, and white clay prepared by Yan Mansion itself, there is no possibility of poisoning—and there is no need for that, Yan Yundang himself knows the severity of pernicious anemia.
"give it to me."
Yan Yundang took the bottle, "Can you take it orally?"
"You can take it orally."
Li Ang nodded and said: "This medicine needs to be taken for life, once a day for seven days. After seven days, it should be taken every half month, and any procedures cannot be changed.
If it takes effect successfully, you will feel significantly better within two or three days, your appetite will increase, and your tongue pain will subside. "
Oral vitamin B12 can also greatly relieve pernicious anemia, but the effect is not as good as intramuscular injection, so it must be taken in greater quantities to prevent the recurrence of anemia.
"Do you take it for life?"
Yan Yundang grinned, "I don't know how many years this old man can live."
After finishing speaking, he raised the bottle and drank it down in a proud gulp, wiping the corners of his mouth.
"Father,"
Yan Lin asked subconsciously: "What's the smell?"
"What else could it smell like, the smell of blood and water."
Yan Yun glanced at his son, and muttered: "It's better than expected.
correct,"
He turned around and looked at Li Ang, "Little Doctor Li, I heard that you were sentenced by Xi Yangyu to be unable to cultivate for life?"
Putting vitamin b12 in bran feed can indeed promote the rapid development of piglets.
In addition, py pushes the book.
Author of "The First Cause of the Worlds": Pei Tugou
(end of this chapter)